Exosomes have emerged as pivotal players in intercellular communication, disease biomarkers, and therapeutic applications. However, their isolation from complex biological fluids remains a significant challenge due to their small size (30-150 nm) and similar density to other extracellular vesicles. Traditional methods like ultracentrifugation are time-consuming, require specialized equipment, and often yield impure preparations with compromised exosome integrity. Our exosome magnetic bead services provide a revolutionary solution, leveraging the power of immunoaffinity-based magnetic separation to offer rapid, specific, and gentle isolation of exosomes. Whether you are exploring disease mechanisms, developing diagnostics, or engineering exosome-based therapeutics, our services deliver the purity and reproducibility essential for groundbreaking discoveries.
Figure 1. Magnetic Bead.
Overview
Exosome magnetic bead technology utilizes superparamagnetic beads conjugated with antibodies against exosome surface markers. When mixed with a sample, these beads specifically bind to exosomes expressing the target antigen. Under a magnetic field, the bead-exosome complexes are isolated efficiently, while contaminants are removed through washing. This method outperforms conventional techniques by combining high specificity with minimal mechanical damage, ensuring intact and functional exosomes ideal for downstream applications like RNA sequencing, proteomics, or functional studies. Our service specializes in tailoring this technology to your specific research or clinical needs, overcoming the limitations of generic kits.
Our Services
We offer a comprehensive suite of services to support every stage of your exosome research, from isolation to downstream analysis.
Exosome Isolation and Purification Service
This is the core of our offering. We provide end-to-end services for the isolation of exosomes from a wide variety of biological fluids, including:
- Plasma/Serum: For biomarker discovery and liquid biopsy applications.
- Urine: A non-invasive sample source for diagnosing kidney diseases and other conditions.
- Cell Culture Supernatant: For fundamental research on cell-to-cell communication and exosome biology.
- Saliva, CSF, and other biofluids: We can also develop custom protocols for more unique or challenging sample types.
We use a panel of specific antibodies to target common exosome markers, ensuring high-purity isolation with minimal contamination from other extracellular vesicles or proteins.
Subpopulation-Specific Exosome Isolation
Not all exosomes are created equal. Our service allows you to isolate specific exosome subpopulations based on their surface markers. For example, by targeting a tumor-specific marker, we can enrich for cancer-derived exosomes from a patient's plasma sample. This is a critical step for developing highly specific and sensitive diagnostic assays.
Exosome Characterization and Downstream Analysis
After isolation, we provide comprehensive services to characterize the purified exosomes and analyze their cargo. This includes:
- Nanoparticle Tracking Analysis (NTA): To determine the size distribution and concentration of the isolated vesicles.
- Western Blotting: To confirm the presence of exosomal markers (e.g., CD9, TSG101) and other target proteins.
- Nucleic Acid Extraction: For the isolation of exosomal RNA (miRNA, lncRNA, mRNA) and DNA for sequencing or qPCR analysis.
- Protein Profiling: We can perform mass spectrometry-based proteomics to identify and quantify the protein cargo of the exosomes.
Custom Antibody Conjugation
If your research requires targeting a unique or novel exosome marker, we offer a custom conjugation service. We can efficiently immobilize your proprietary antibody onto our high-performance magnetic beads, creating a tailored tool for your specific application. Our team will ensure the conjugation process does not compromise the antibody's binding affinity or the bead's performance.
Application
Our services are applicable to a broad range of research and clinical fields.
- Liquid Biopsy: Non-invasive detection and monitoring of cancer, neurological diseases, and other conditions by analyzing exosomal cargo.
- Drug Delivery and Therapeutics: Using exosomes as a natural delivery system for small molecules, RNA, or proteins.
- Biomarker Discovery: Identifying novel biomarkers in exosomes for early disease diagnosis and prognosis.
- Fundamental Biology: Studying the role of exosomes in cell-to-cell communication, inflammation, and immune responses.
Our Process
Our approach is highly collaborative, ensuring the final product meets your exact specifications:
Synthesis and
functionalization
Characterization and
validation
Quality assurance
and delivery
Our Advantages
High Purity and Yield
Our optimized protocols and high-quality reagents ensure that you get a highly pure and concentrated exosome sample. This is critical for downstream analysis, as it minimizes background noise and allows for more accurate and sensitive results.
Speed and Efficiency
Magnetic bead-based isolation is significantly faster than ultracentrifugation. This efficiency allows for a higher sample throughput, making it ideal for large-scale studies and clinical applications.
Preserved Integrity
The gentle nature of our method ensures that the exosomes remain intact and their biological activity is preserved. This is vital for applications where the function of the exosome or its cargo needs to be studied.
Our exosome magnetic bead services are a game-changer for anyone working with these valuable biological vesicles. By providing a fast, efficient, and reliable method for exosome isolation and analysis, we empower you to unlock new discoveries and accelerate the development of groundbreaking diagnostics and therapeutics. We are committed to delivering the highest quality service and working as your partner to achieve your research goals. Let us help you navigate the complexities of exosome science, so you can focus on the bigger picture.
1. What is the minimum and maximum sample volume you can process?
We can handle a wide range of sample volumes, from microliters for precious samples to several milliliters for large-scale isolation. Please contact us to discuss your specific needs.
2. What exosome markers can you target for isolation?
We routinely use antibodies against common exosome markers like CD9, CD63, and CD81. We can also develop custom protocols for other markers based on your research needs.
3. What downstream analysis services do you provide?
We offer a comprehensive suite of downstream services, including NTA for particle analysis, nucleic acid extraction for RNA/DNA sequencing, Western Blotting for protein confirmation, and mass spectrometry-based proteomics.
4. How do you ensure the purity of the isolated exosomes?
We use a combination of specific antibody-based capture and optimized wash protocols to minimize non-specific binding. We can also provide a negative control and a characterization report to demonstrate the purity of your sample.
