CD Bioparticles' magnetic bead nucleic acid conjugation services are a convenient and dependable approach for conjugating nucleic acids (DNA, RNA, and their analogs) to magnetic beads. Our magnetic bead nucleic acid conjugation services platform, which is grounded on our background in material science and bioconjugation techniques, creates high-quality, high-performance nucleic acid-magnetic bead conjugates to meet a wide range of research, diagnostic, and commercial needs. Whether you need nucleic acid purification, identification, or delivery conjugates, we offer services that provide consistent quality, selectivity, and productivity to assist you in getting your work done.
Figure 1. Magnetic Bead Nucleic Acid Conjugation Services.( Kashyap D, et al. 2024)
Overview of Magnetic Bead Nucleic Acid Conjugation Technology
CD Bioparticles have a team of experts who have a deep understanding of magnetic bead surface chemistry (carboxyl, amine, streptavidin modification, etc.) and nucleic acid experience. They can help you choose the most suitable coupling method, whether it is covalent coupling (amine thiol chemistry) or affinity based coupling (biotin streptavidin interaction). We guarantee that the obtained conjugate has the necessary functions of nucleic acid and magnetic beads, including magnetic responsiveness and long-term stability, allowing you to focus on the key goals of the project without worrying about coupling obstacles.
Applications
CD Bioparticles' magnetic bead nucleic acid conjugation services create nucleic acid-magnetic bead conjugates that can be employed in a variety of applications, including the following:
Gene Therapy and Delivery
Conjugates, on the other hand, can be used to deliver nucleic acids to target cells or tissues. For example, siRNA-magnetic bead conjugates can be more precisely delivered to target cells, increasing transfection efficiency and reducing off-target effects in gene silencing research.
Biosensing and Research
In both academic and industrial research, conjugates can be used to study nucleic acid interactions (such as protein-DNA binding) and to build biosensors for real-time nucleic acid detection in applications including drug discovery, biomarker discovery, or environmental testing.
Our Services
CD Bioparticles provides magnetic bead nucleic acid conjugation services for a variety of requirements:
Surface functionalization
Surface functionalization
We provide various active functional groups on the surface of magnetic beads, including
- Amine
- Carboxyl
- Thiol
- Aldehyde
- Active hydrogen functional groups
Customized coupling of nucleic acid and magnetic beads
We provide customized coupling services for DNA, RNA, oligonucleotides, and other nucleic acid molecules. Our team ensures optimal bonding efficiency and stability through advanced surface functionalization technology.
Quality control and characterization
Each customized conjugate undergoes rigorous lateral flow immunoassay (LFIA) testing to ensure quality and consistency. We also provide detailed characterization reports, including size distribution, zeta potential, and binding ability.
Scalability and Customization
Whether you need small-scale research samples or large-scale production for commercialization, CD Bioparticles can provide customized solutions that meet your specific requirements. We support non GMP and GMP level production.
Support downstream applications
We work closely with our customers to ensure that conjugated magnetic beads are suitable for their intended applications, such as PCR, qPCR, sequencing, and microarray analysis
Workflow
Synthesis and Optimization
| Process | Descriptions |
|---|
| Preparation of magnetic beads | We first select the appropriate size and functional group of magnetic beads according to the customer's requirements. Beads are pre functionalized with various active groups to promote nucleic acid binding. |
| Nucleic acid coupling | Using optimized schemes to couple selected nucleic acid molecules onto magnetic beads. This process involves activating the bead surface, coupling nucleic acids, and blocking any unreacted sites to prevent non-specific binding. |
| Conjugation Reaction | Based on the materials we use, we use the appropriate conjugation method: e.g., carbodiimide chemistry for coupling carboxyl beads and amine-modified nucleic acids, or streptavidin-biotin affinity for non-covalent binding. Reactions are monitored in real time for optimization. |
| Washing & Blocking | Following conjugation, unbound nucleic acids are removed by magnetic separation and washing in appropriate buffers. Unspecific binding sites on the beads are blocked (e.g., with BSA or DNA-free blockers) to reduce background in the desired application. |
| Quality Testing | All conjugates are tested as described in "Our Service" to verify their quality. If the conjugate is up to par, we move forward with the order. If the results are not as anticipated, we will rework and re-conjugate the beads. |
Summary
CD Bioparticles is your trusted partner for magnetic bead nucleic acid binding services, providing high-performance solutions for research, diagnosis, and treatment. Our expertise ensures reliable and efficient fulfillment of your specific needs.
1. Can you use client-provided magnetic beads or nucleic acids?
We can, indeed. If you give us magnetic beads, we will first test to see whether they are appropriate for our conjugation procedures. We will test nucleic acids provided by the client for purity and quality before conjugating them to guarantee that they will produce the greatest possible results.
2. Do you provide technical support for conjugate performance issues?
Yes, we do. Following delivery, our team will provide support, including advice on how to use the conjugate in your application. We will also help you with any issues that may occur (such as low binding efficiency), determining the possible causes (storage conditions, assay parameters, etc. ), and suggest solutions if necessary.
3. What quality control measures are in place?
We employ a comprehensive suite of quality control measures, including spectrophotometric quantification of conjugated nucleic acids, assessment of bead integrity and aggregation (DLS), evaluation of binding capacity, and functional validation where applicable.
Reference
- Kashyap D, Booth M J. Nucleic Acid Conjugates: Unlocking Therapeutic Potential. ACS Bio & Med Chem Au, 2024, 5(1): 3-15.
