Magnetic beads (or superparamagnetic particles) are a common and popular tool in molecular biology. These iron oxide-based beads can be suspended in solution and easily manipulated with an external magnetic field. Magnetic beads can be used in a wide variety of applications including antibody purification, nucleic acid extraction and purification, and cell separation. There are some limits to how useful magnetic beads are and they come down to the chemistry of the surface and how that surface has been functionalized. At CD Bioparticles, we can custom conjugate a wide variety of biomolecules and metals onto magnetic beads to address a range of applications. Our scientists and engineers have extensive experience in nanoparticle synthesis and surface modification as well as an in-depth understanding of the different conjugation technologies available to customise magnetic beads.
Figure 1. Magnetic Bead Metal Conjugation Services.
Introductions
CD Bioparticles is an international nanoparticle, microparticle, and their coating manufacturer. We have over 10 years of experience, and a key focus of our business is to work directly with our customers to provide the most fit-for-purpose products. Our core competencies include magnetic bead conjugation and metal nanoparticle conjugation for all forms of molecular detection, imaging, drug delivery and immunoassay development. These include lateral flow assay development as well as digital PCR assays. We work with researchers, diagnostic developers, and pharma companies all around the world.
Applications
Cell Biology and Separation
Magnetic beads can be used to isolate specific cell populations or organelles from heterogeneous mixtures. This can be useful in stem cell research, cancer biology, and immunotherapy development.
Nucleic Acid Analysis
Magnetic beads can be used for the extraction, purification, and amplification of DNA and RNA. They can be coated with oligonucleotides or enzymes to facilitate PCR, qPCR, and sequencing workflows.
Drug Delivery System
Metal nanoparticles, such as gold or silver nanoparticles, can be bound to magnetic beads to create advanced drug delivery systems. These systems can offer enhanced targeting, controlled release, and improved therapeutic efficacy.
Our Services
CD Bioparticles offers a comprehensive suite of magnetic bead and metal nanoparticle conjugation services, tailored to meet the unique needs of each client. Our services include:
Customized combination
We can combine various metals (such as gold, silver, iron, platinum, and copper) and metal nanoparticles onto different types of magnetic beads (including iron oxide magnetic beads, polymer coated magnetic beads, and functionalized magnetic beads) according to customer requirements. We can also adjust parameters such as metal loading, conjugation efficiency, and particle size to achieve the desired performance of the conjugate.
Quality Control
We implement strict quality control measures to ensure the quality and performance of the conjugate. Our quality control testing includes measurements of particle size and size distribution, zeta potential, metal content, magnetic responsiveness, and binding efficiency. We provide customers with detailed quality control reports.
Batch Production
We have the ability to mass produce magnetic bead metal conjugates to meet the requirements of industrial scale applications. Our production process has been optimized for efficiency and consistency, ensuring that the conjugates meet the same high standards regardless of batch size.
Scale-up and commercialization support
We can provide both small-scale and large-scale production services, and offer GMP-grade manufacturing for commercial production.
Workflow
Synthesis and Optimization
| Process | Descriptions |
|---|
| Experimental Design | According to the customer's requirements, our team of scientists has designed a detailed experimental plan, including the selection of conjugation methods, reaction conditions, and quality control parameters. |
| Magnetic Bead Preparation | Proper cleaning, activation (if necessary), and characterization of selected magnetic beads to ensure their quality and suitability. |
| Metal Conjugation | The conjugation reaction is carried out under optimized conditions. We monitor the reaction progress to ensure that the desired conjugation efficiency and metal loading are achieved. |
| Purification | After the conjugation reaction, the conjugates are purified to remove unreacted metals, by - products, and other impurities. This purification step is crucial for ensuring the purity and performance of the conjugates. |
| Delivery and Support | We deliver the conjugates to the client along with a detailed report that includes information about the conjugation process, quality control results, and storage conditions. We also provide technical support to assist the client in using the conjugates. |
Summary
CD Bioparticles has been at the forefront of magnetic bead conjugation technology development and optimization. Our team of experienced scientists has extensive expertise in various conjugation methods and techniques, enabling us to produce high-performance magnetic bead metal conjugates with controlled conjugation efficiency, metal loading, and particle size.
1. What is the shelf life of the magnetic bead metal conjugates?
The shelf life of the conjugates depends on the storage conditions. When stored at 4°C in a suitable buffer, the conjugates can remain stable for 6 - 12 months. We provide specific storage instructions with each delivery.
2. Do you offer samples before bulk production?
Yes, we can provide small - scale samples for the client to test. This allows the client to evaluate the performance of the conjugates before proceeding with bulk production.
3. Can you conjugate multiple metals to the same magnetic bead?
Yes, we can conjugate multiple metals to the same magnetic bead, depending on the client's needs. However, this may require additional optimization of the conjugation process.
4. What is the shelf life of the magnetic bead metal conjugates?
The shelf life of the conjugates depends on the storage conditions. When stored at 4°C in a suitable buffer, the conjugates can remain stable for 6 - 12 months. We provide specific storage instructions with each delivery.
