At CD Bioparticles, we are at the forefront of single-cell analysis, providing cutting-edge Magnetic Bead Assisted Single-Cell Sorting Platform Development services. We empower researchers in immunology, oncology, and drug discovery to overcome the limitations of traditional cell sorting methods. By harnessing the specificity and versatility of magnetic bead technology, we develop customized platforms for the highly precise, gentle, and cost-effective isolation of target cell populations. Our solutions are designed to integrate seamlessly into your workflow, delivering pristine single-cells ready for downstream genomic, transcriptomic, or proteomic analysis.
Introductions
The ability to isolate pure, viable single cells is the foundation of modern single-cell biology. While fluorescence-activated cell sorting (FACS) is widely used, it can be costly, impose shear stress on cells, and require complex instrumentation. Magnetic-activated cell sorting (MACS) offers a gentle alternative but often lacks the precision for true single-cell resolution. CD Bioparticles bridges this gap. We develop advanced platforms that combine the robust positive selection of magnetic beads with innovative microfluidic or microwell architectures, enabling the reliable isolation of individual cells based on specific surface markers, with minimal stress and maximal recovery.
Technology Overview
Our platform is built upon a foundation of high-performance magnetic beads and sophisticated engineering to achieve precise single-cell sorting.
Key Technical Components:
- High-Performance Magnetic Beads: We utilize or develop nano- to micro-sized magnetic beads with superior colloidal stability and minimal non-specific binding. These beads can be conjugated with a vast array of antibodies, ligands, or other capture molecules to target specific cell surface antigens.
- Integrated Microfluidic/Microwell Chips: Our custom-designed chips create defined micro-environments that confine individual cells. The integration of micro-magnets or an external magnetic field allows for the highly localized capture and retention of bead-bound target cells within these individual compartments.
- Gentle Cell Handling System: The workflow is optimized to minimize mechanical and shear forces, ensuring high cell viability and integrity post-sort, which is critical for subsequent single-cell sequencing or culture.
- Automation and Visualization Interface: Our platforms can be designed with compatibility for automation and often include imaging capabilities to confirm single-cell occupancy and sorting efficiency in real-time.
Our Services
We offer end-to-end development services to create a bespoke single-cell sorting solution for your unique research needs:
- Custom Assay Development: We design the entire workflow, from sample preparation (whole blood, PBMCs, tissue dissociates) to single-cell isolation, tailored for your target cell type.
- Bead Conjugation & Validation: We conjugate your specific antibodies onto our optimal magnetic beads and rigorously validate their specificity and functionality.
- Chip Design & Fabrication: Our engineering team designs and prototypes microfluidic or microwell chips optimized for your application, cell size, and throughput requirements.
- Platform Integration & Optimization: We integrate the magnetic separation unit with the chip system and fine-tune all parameters, including flow rates, magnetic field strength, and buffer conditions.
- Performance Validation & Support: We provide comprehensive data on sorting purity, efficiency, cell viability, and compatibility with downstream applications like scRNA-seq.
Applications
Our Magnetic Bead Assisted Single-Cell Sorting Platform is ideal for a wide range of advanced research applications:
- Single-Cell Genomics and Transcriptomics: Isolate pure populations of rare cells (e.g., circulating tumor cells, specific T-cell clones) for scRNA-seq with minimized background.
- Cancer Research: Identify and isolate cancer stem cells or heterogeneous subpopulations from tumors for functional studies.
- Immunology: Precisely sort immune cell subtypes (e.g., antigen-specific B cells, Tregs) to study immune responses and develop cell therapies.
- Stem Cell Research: Isolate specific stem cell populations based on surface markers for regenerative medicine applications.
- Microbiology: Separate individual bacteria or fungi for antimicrobial resistance studies or microbiome analysis.
Our Process
Our approach is highly collaborative, ensuring the final product meets your exact specifications:
Synthesis and functionalization
Characterization and validation
Quality assurance and delivery
Our Advantages
Deep Technical Expertise
CD Bioparticles boasts decades of experience in magnetic particle synthesis, surface modification, and bioconjugation. Our proprietary technologies and in-house R&D capabilities enable us to develop high-performance magnetic beads with tailored properties.
End-to-End Capabilities
We recognize that every client's needs are unique. Our services cover full customization—from magnetic bead design and ligand conjugation to workflow optimization and platform integration—adapting to specific target proteins, sample types, and experimental requirements.
Rigorous Quality Assurance
All our magnetic beads and platform components undergo strict quality control (QC) at every stage—from raw material selection and synthesis to conjugation and final validation. Our commitment to quality guarantees that you receive a reliable, robust platform that accelerates your project progress.
Single-cell sorting is critical for unlocking insights into cellular heterogeneity, but traditional methods often compromise cell quality or flexibility. CD Bioparticles' Magnetic Bead Assisted Single-Cell Sorting Platform Development combines advanced magnetic bead technology with tailored workflows to deliver high-viability, high-purity sorting—supporting your research from basic science to clinical translation. Whether you need a prototype for academic research or a scalable platform for biopharma production, our team of scientists works closely with you to ensure the platform meets your performance goals and timeline. Partner with CD Bioparticles to accelerate your single-cell research and innovation.
